recombinant human cd21  (Sino Biological)

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: SEM (A–C) and AFM (D–F) images of (A, D) CD21@AuNP, (B,E) BSA@CD21@AuNP, and (C, F) gp350@BSA@CD21@AuNP.

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques:

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: (A) FTIR and (B) UV spectra for the characterization of the developed colorimetric EBV biosensor. Step-by-step FTIR and UV spectra of the biosensor during gp350 detection: (a) 2.1 mL of AuNPs; (b) 2.1 mL of AuNPs + 400 μL of CD21 at 20 ng·mL –1 ; (c) 2.1 mL of AuNPs + 400 μL of CD21 at 20 ng·mL –1 + 100 μL of 1% BSA; and (d) 2.1 mL of AuNPs + 400 μL of CD21 at 20 ng·mL –1 + 100 μL of 1% BSA + 50 ng·mL –1 of gp350.

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques:

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: (A) UV spectra and (B) bar chart that belong to the optimization of the CD21–gp350 interaction incubation temperature. (a) BSA@CD21@AuNP, (b) BSA@CD21@AuNP + 50 ng·mL –1 gp350 for 4 °C, (c) 25 °C, (d) 37 °C, and (e) 45 °C incubation temperature.

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques: Incubation

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: (A) UV spectra and (B) Excel plot that belong to the optimization of the CD21–gp350 interaction incubation time. (a) BSA@CD21@AuNP, (b) BSA@CD21@AuNP + 50 ng·mL –1 gp350 for 15 min, (c) 30 min, (d) 60 min, and (e) 90 min incubation time.

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques: Incubation

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: Analytical characteristics of the developed colorimetric EBV biosensors. (A) UV spectra of (a) BSA@CD21@AuNP, (b) 10 ng·mL –1 , (c) 25 ng·mL –1 , (d) 50 ng·mL –1 , (e) 80 ng·mL –1 , and (f) 100 ng·mL –1 gp350 additions. (B) Calibration plot of increasing concentrations of gp350 from 10 ng·mL –1 to 100 ng·mL –1 .

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques:

Journal: Analytical Chemistry

Article Title: Early Detection of Multiple Sclerosis Using Spectroscopic Epstein–Barr Virus Biosensors Based on Infection Mechanism Mimicry

doi: 10.1021/acs.analchem.5c03964

Figure Lengend Snippet: UV spectra demonstrating the absorbance difference of a 1:1 ratio of (a) BSA@CD21@AuNP, (b) gp350 @BSA@CD21@AuNP, and (c) A27L + KMP11 + gp350 @BSA@CD21@AuNP.

Article Snippet: Recombinant human CD21 and recombinant EBV gp350 proteins were purchased from Sino Biological Inc. All reagents and chemicals employed in this study were analytical grade and used without further purification.

Techniques:

Journal: PLoS ONE

Article Title: Interaction of Staphylococci with Human B cells

doi: 10.1371/journal.pone.0164410

Figure Lengend Snippet: A) FITC-labeled S . epidermidis (strain 1457) was opsonized with NHS, heat-treated NHS (HT Tx), or DPBS (unopsonized control) and binding of B cells to S . epidermidis was assessed by flow cytometry. B) FITC-labeled S . epidermidis was opsonized with NHS, NHS treated with cobra venom factor (+CVF), NHS treated with FUT-175 (+FUT-175), HT Tx, or DPBS and binding of B cells to S . epidermidis was assessed by flow cytometry (60 min time point). Data in panels A and B are the mean ± SEM of 5 separate experiments using different blood donors. C) FITC-labeled S . epidermidis was combined with 5 μg anti-human CD35 (+αCR1), CD21 (+αCR2), CD11b (+αCR3), CD11c (+αCR4), or isotype IgG 2 control (isotype) mAbs and binding to B cells was determined using flow cytometry (30 min time point). D) FITC-labeled S . epidermidis was combined with 4 μg of soluble recombinant CD35 (+rCR1), CD21 (+rCR2), CD11b (+rCR3), CD11c (+rCR4), or DPBS and binding to B cells was determined using flow cytometry (60 min time point). Data in panels C and D are the mean ± SEM of at least 3 separate experiments using different blood donors. Experiments in panels A-D were performed using 1 × 10 6 purified human peripheral blood mononuclear cells (PBMCs) and 1 × 10 6 CFUs S . epidermidis . B cells were identified as the CD19 + leukocyte population of PBMCs. ** P <0.01, *** P <0.001, and **** P <0.0001 versus DPBS controls for panels A and D, NHS for panel B, or isotype IgG 2 for panel C. Statistics were determined by using a repeated-measures one-way ANOVA and Dunnett’s post-test.

Article Snippet: For assays that used recombinant complement receptors, 1 × 10 6 CFUs of opsonized FITC-labeled S . epidermidis were combined with 4 μg of recombinant CD21, CD35, CD11b, or CD11c (R&D Systems) and incubated on ice for 10 min prior to being added to purified human PBMCs.

Techniques: Labeling, Control, Binding Assay, Flow Cytometry, Combined Bisulfite Restriction Analysis Assay, Recombinant, Purification

Journal: PLoS ONE

Article Title: Interaction of Staphylococci with Human B cells

doi: 10.1371/journal.pone.0164410

Figure Lengend Snippet: A) FITC-labeled Newman wild-type or isogenic spa deletion strains (NewmanΔ spa ) (each at 5 × 10 5 CFU/mL) were incubated in heparinized human blood and binding of bacteria to B cells was determined by flow cytometry. B) FITC-labeled USA400 wild-type or isogenic saeR/S (USA400Δ saeR/S ) or agr (USA400Δ agr ) deletion strains (each at 5 × 10 5 CFU/mL) were incubated in heparinized human blood and binding of bacteria to B cells was determined by flow cytometry. Panels A and B are the mean ± SEM of 3 separate experiments using different blood donors. Panels C-E show the relative distribution of CD66 High granulocytes, CD14 + monocytes, CD19 + lymphocytes, and CD3 + lymphocytes associated with USA400 (C) , USA400Δ saeR/S (D) , and USA400Δ agr (E) at 40 min. Data were collected from experiments described in panel B. F,G) Association and internalization of USA400, USA400Δ saeR/S , or USA400Δ agr by purified human B cells (F) or monocytes (G) was determined 80 min after addition of bacteria (1 × 10 6 CFU/mL) by using immunofluorescence microscopy. H) FITC-labeled USA400Δ saeR/S (1 × 10 6 ) was combined with 5 μg anti-human CD35 (+αCR1), CD21 (+αCR2), CD11b (+αCR3), CD11c (+αCR4), or isotype IgG 2 control (Isotype Ab) mAbs and binding to B cells was determined using flow cytometry (30 min time point). Panels F and G are the mean ± SEM of 4 separate experiments using different blood donors. Panel H is the mean ± SEM of 5 separate experiments using different blood donors. Statistics were determined by using a repeated-measures one-way ANOVA and Dunnett’s post-test. For panel F, ** P <0.05 versus the wild-type strain (USA400). For panel H, *** P <0.001 and **** P <0.0001 versus isotype IgG 2 .

Article Snippet: For assays that used recombinant complement receptors, 1 × 10 6 CFUs of opsonized FITC-labeled S . epidermidis were combined with 4 μg of recombinant CD21, CD35, CD11b, or CD11c (R&D Systems) and incubated on ice for 10 min prior to being added to purified human PBMCs.

Techniques: Labeling, Incubation, Binding Assay, Bacteria, Flow Cytometry, Purification, Immunofluorescence, Microscopy, Control